Characterization of Tomato Brown Rugose Fruit Virus (ToBRFV) Detected in Czech Republic

Tomato is the most consumed vegetable in world. The tomato brown rugose fruit virus (ToBRFV) an important destructive that damages tomatoes and peppers with significant economic impact. detection characterization of this viral pathogen were evaluated at molecular morphological level. isolate was purified inoculated on pepper plants. Small RNAs sequenced both plants profiles compared. complete genome obtained, microRNA (miRNA) unveiled by small RNA sequencing. Symptoms caused also described morphology observed transmission electron microscopy. Our results contribute to further understanding role miRNAs ToBRFV pathogenesis, which may be crucial for disease symptom development peppers.